THE CONSTRUCTION OF THE WORLD-WIDE WEB-ACCESSIBLE MYOCARDIAL 2-DIMENSIONAL GEL-ELECTROPHORESIS PROTEIN DATABASE HEART-2DPAGE - A PRACTICAL APPROACH

The construction of the two-dimensional electrophoresis (2-DE) gel pro tein database ''HEART-2DPAGE'' for human heart proteins, accessible vi a the World Wide Web (WWW), is described. This database provides the o pportunity to retrieve descriptive information interactively by mouse clicking on a protein spot within a 2-DE gel image or to retrieve the position of a protein if its protein name or a search expression is gi ven. The realization of searching for proteins, the creation of clicka ble spots, marked by crosses or textual information, and interactive v iewing opportunities are discussed from a software-oriented point of v iew. The database takes into account that some human heart proteins ar e chamber specific. Several Common Gateway Interface (CGI) scripts are described and some practical experience is discussed.