EXOCRINE PANCREATIC SECRETIONS IN GROWING PIGS FED DIETS CONTAINING FISH-OIL, RAPESEED OIL OR COCONUT OIL

Two experiments were performed to study the effect-of feeding diets co ntaining oils with different fatty acid composition on exocrine pancre atic secretions in growing pigs using two different methods to collect pancreatic juice. In the first experiment, three barrows (initial wei ght 37 kg) were fitted with a pancreatic pouch re-entrant cannula. An isolated pouch was prepared where the pancreatic duct enters the duode num. In the second experiment, also using three barrows (initial weigh t 32 kg), a catheter was inserted into the pancreatic duct. Three whea t starch and fish meal-based diets were formulated to contain either 1 5 g fish oil, rapeseed oil or coconut oil/100 g. In both experiments, the diets were fed according to a 3 times 3 Latin square design. The v olume of pancreatic juice secreted, pH and secretion of bicarbonate, p rotein, amylase, trypsin, lipase and colipase were not significantly a ffected by the diets in the first experiment. In the second experiment , chymotrypsin secretion was significantly greater in pigs fed the coc onut oil diet, and secretion of carboxyl ester hydrolase was significa ntly higher in pigs fed the fish oil diet. When compared qualitatively , pigs in Experiment 2 secreted more pancreatic juice; the pancreatic juice had a higher pH, and trypsin, carboxyl ester hydrolase and colip ase secretions were substantially higher whereas amylase secretion was lower than for pigs in Experiment 1. The fatty acid composition of th e different oils had minor effects on exocrine pancreatic secretion in growing pigs. However, there were considerable differences between th e two surgical methods used to collect pancreatic juice, and these dif ferences may be explained by physiological changes induced by the two methods.