ONE-STEP PCR MEDIATED STRATEGY FOR THE CONSTRUCTION OF CONDITIONALLY EXPRESSED AND EPITOPE-TAGGED YEAST PROTEINS

Authors
LAFONTAINE D TOLLERVEY D
Citation
D. Lafontaine et D. Tollervey, ONE-STEP PCR MEDIATED STRATEGY FOR THE CONSTRUCTION OF CONDITIONALLY EXPRESSED AND EPITOPE-TAGGED YEAST PROTEINS, Nucleic acids research, 24(17), 1996, pp. 3469-3471
Citations number
9
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
24
Issue
17
Year of publication
1996
Pages
3469 - 3471
Database
ISI
SICI code
0305-1048(1996)24:17<3469:OPMSFT>2.0.ZU;2-Q
Abstract
With the availability of the complete yeast genomic sequence, techniqu es which allow the rapid functional analysis of genes of interest are of increasing importance. Here we report a technique which allows the initial characterisation of genes of interest, through the constructio n of conditionally expressed mutations for functional analyses and the generation of epitope-tagged fusion proteins for immuno-localisation and immuno-purification, entirely by PCR.