POLYCLONAL ENZYME-IMMUNOASSAY SYSTEM FOR DETECTING HANTAVIRUS ANTIGENS - ASSESSING THE SPECIFICITY USING MONOCLONAL-ANTIBODIES AND POLYMERASE CHAIN-REACTION

Authors
IVANOV AP DEKONENKO YP SHUTKOVA TM DZAGUROVA TK TKACHENKO YA
Citation
Ap. Ivanov et al., POLYCLONAL ENZYME-IMMUNOASSAY SYSTEM FOR DETECTING HANTAVIRUS ANTIGENS - ASSESSING THE SPECIFICITY USING MONOCLONAL-ANTIBODIES AND POLYMERASE CHAIN-REACTION, Voprosy virusologii, 41(3), 1996, pp. 110-112
Citations number
10
Categorie Soggetti
Virology
Journal title
Voprosy virusologii → ACNP
ISSN journal
05074088
Volume
41
Issue
3
Year of publication
1996
Pages
110 - 112
Database
ISI
SICI code
0507-4088(1996)41:3<110:PESFDH>2.0.ZU;2-2
Abstract
Genus-specific monoclonal antibodies to nucleocapsid protein of Pumala virus and polymerase chain reaction were used to assess the specifici ty of a previously described polyclonal (based on human IgG to Pumala hantavirus) enzyme immunoassay (EIA) system for detecting hantavirus a ntigens. Parallel testing of crude lung suspensions From wild rodents trapped in different regions of Russia for hantavirus antigens and RNA showed 100% coincidence of the results of three tests. These data. in dicate a high specificity of polyclonal EIA system and its efficacy fo r screening of natural samples.