POOLED HUMAN-IMMUNOGLOBULINS CONTAIN ANTIIDIOTYPES WITH REACTIVITY AGAINST THE SLE-ASSOCIATED 4B4 CROSS-REACTIVE IDIOTYPE

Citation
F. Dekeyser et al., POOLED HUMAN-IMMUNOGLOBULINS CONTAIN ANTIIDIOTYPES WITH REACTIVITY AGAINST THE SLE-ASSOCIATED 4B4 CROSS-REACTIVE IDIOTYPE, Clinical and experimental rheumatology, 14(6), 1996, pp. 587-591
Citations number
27
Categorie Soggetti
Rheumatology
ISSN journal
0392856X
Volume
14
Issue
6
Year of publication
1996
Pages
587 - 591
Database
ISI
SICI code
0392-856X(1996)14:6<587:PHCAWR>2.0.ZU;2-X
Abstract
Objective: To identify anti-idiotype antibody activity against a regul atory anti-Sm idiotype (4B4) in pooled human IgG (IVIG). Methods: ELIS A were used to determine anti-idiotype antibody activity in IVIG.Resul ts: Anti-idiotype antibody activity to 4B4 was detected in IVIG. The a nti-idiotypic activity in IVIG could be inhibited by antigen, Sm, and rabbit anti-idiotypic antiserum. The idiotpic binding activity was pre dominately directed against the 4B4 V-H region. Conclusion: IVIG conta ins antibodies against a lupus-associated crossreactive idiotype. Thes e antibodies represent Ab(2 beta) type antibodies. The anti-idiotypic activity in IVIG may be relevant to the efficacious use of IVIG in tre ating rheumatic diseases.