PRODUCTION OF HUMAN GLICENTIN IN ESCHERICHIA-COLI

The gene encoding for human glicentin comprising 69 amino acid residue s, a major form of gut glucagon, was designed and chemically synthesiz ed based on the amino acid sequence deduced from the sequence of human preproglucagon. The gene was ligated to an expression vector pET-3a a nd expressed in Escherichia coli. The product thus obtained had an ext ra methionine at the N-terminus of glicentin molecule. Subsequently, t he gene fragment encoding the first 10 amino acids of gene 10 protein of T7 phage (s10 peptide) was introduced into N-terminus of the glicen tin gene to express a s10-glicentin fusion protein. The treatment of t he ensuing fusion protein with cathepsin C produced human glicentin su ccessfully.