CLONING AND EXPRESSION OF CDNA-ENCODING A HUMAN UBIQUITIN-CONJUGATINGENZYME SIMILAR TO THE DROSOPHILA BENDLESS GENE-PRODUCT

eDNA encoding a novel human ubiquitin-conjugating enzyme has been clon ed from an epidermoid carcinoma KB cDNA library. This clone encodes a protein of 152 amino acids with a calculated M(r) of 17,137. The amino acid sequence showed 80% identity with the Drosophila's bendless gene product (ubiquitin-conjugating enzyme E2). The corresponding transcri pts ape highly expressed in heart, skeletal muscle, and testis. The pr oduct expressed in Escherichia coli exhibited the ability to form a th iol ester linkage with ubiquitin in a ubiquitin-activating enzyme E1-d ependent manner. These results suggest that the obtained cDNA encodes a novel human E2 which may be involved in protein degradation mainly i n the muscles and testis.