ISOLATION OF FUNCTIONALLY INTACT PIG RETINA MITOCHONDRIA

Two alternative methods for the isolation of functionally intact mitoc hondria from pig retina were evaluated by means of differential centri fugation. If ATP and bovine serum albumin were present during the isol ation procedures mitochondria exhibited ADP sensitive respiration and membrane potentials, comparable to values from other tissues. The mito chondria fraction of both methods differed in purity, being almost fre e of synaptosomes when a discontinuous Ficoll gradient centrifugation was used.