An IgG(1) mouse monoclonal antibody (MAb) YPC3 against human pancreati
c carcinoma was generated by a hybridoma technique, When human tissue
sections were examined with immunohistochemical staining, YPC3 MAb rea
cted with 28 of 32 (87.5%) cases of pancreatic carcinoma tissue; it fa
iled to react with all 11 cases of normal pancreas examined, When (TC)
-T-99m-radiolabeled YPC3 MAb was administrated intravenously to the nu
de mice bearing xenograft of pancreatic carcinoma, clear scintigraphic
visualization of tumor xenograft was found with SPECT 24 h after inje
ction, Administration of either YPC3 MAb or LAK cells combined with YP
C3 MAb could inhibit the growth of the xenografts in nude mice. These
results suggest that YPC3 MAb might be an ideal carrier for radioimmun
olocalization and immunotherapy of human pancreatic carcinoma.