GENERATION AND APPLICATION OF MONOCLONAL-ANTIBODY TO HUMAN PANCREATIC-CARCINOMA

An IgG(1) mouse monoclonal antibody (MAb) YPC3 against human pancreati c carcinoma was generated by a hybridoma technique, When human tissue sections were examined with immunohistochemical staining, YPC3 MAb rea cted with 28 of 32 (87.5%) cases of pancreatic carcinoma tissue; it fa iled to react with all 11 cases of normal pancreas examined, When (TC) -T-99m-radiolabeled YPC3 MAb was administrated intravenously to the nu de mice bearing xenograft of pancreatic carcinoma, clear scintigraphic visualization of tumor xenograft was found with SPECT 24 h after inje ction, Administration of either YPC3 MAb or LAK cells combined with YP C3 MAb could inhibit the growth of the xenografts in nude mice. These results suggest that YPC3 MAb might be an ideal carrier for radioimmun olocalization and immunotherapy of human pancreatic carcinoma.