FAT EMULSIONS BASED ON STRUCTURED LIPIDS (1,3-SPECIFIC TRIGLYCERIDES)- AN INVESTIGATION OF THE IN-VITRO INTERACTION WITH PLASMA-PROTEINS

Structured lipids (1,3-specific triglycerides) are new chemical entiti es made by enzymatic transesterification of the fatty acids in the 1,3 -positions of the triglyceride. The purpose of this study was to inves tigate the in vitro interaction of fat emulsions based on either struc tured lipids or vegetable oils with human plasma proteins employing tw o-dimensional polyacrylamide gel electrophoresis (2-D PAGE). The struc tured lipids are triglycerides of the SLS and MLM types, where S is sh ort-chain fatty acids (C-4), M is medium-chain fatty acids (C-8-10) an d L is long-chain fatty acids (C-16-18). The vegetable oil-based fat e mulsions were the commercially available product, Intralipid, and a so ybean oil (LLL) emulsion made de novo identically as the emulsions con taining structured lipids. The SLS emulsion was found to adsorb a diff erent protein pattern than the MLM and LLL emulsions. The protein patt ern of the SLS emulsion was similar to the protein pattern of Intralip id. These findings might explain the in vivo difference in elimination found in another study, where the emulsion based on structured lipids with short-chain fatty acids in the 1,3-positions was removed more sl owly from the general blood circulation compared to emulsions based on lipids with long-chain fatty acids in the 1,3-positions (LLL) (1).