FUNGAL FERMENTATION OF WHEY INCORPORATED WITH CERTAIN SUPPLEMENTS FORTHE PRODUCTION OF PROTEASES

The pattern and the extent of formation of proteases and secretion var ied with the fungus, age and/or the nature of the cosupplement. Additi on of yeast extract induced the best yield of proteases from both Aspe rgillus niger and A. terreus. Proteases from A. niger were highly indu ced by glutamic acid, alanine or albumin, with minor differences, wher eas gelatin, peptone, aspartic acid, casein or acetamide stimulated th e accumulation of proteases in the culture medium of A. terreus. Galac tose stimulated the yield of the enzyme particularly with A, terreus w hile most C-supplements inhibited such processes, more prominently in the presence of cellulose or starch. Incorporation of nicotinic acid a nd wheat bran initiated the maximum productivity of proteases from A. niger and A. terreus, respectively. Co+2 and Cu+2 highly stimulated th e output of proteases from A. niger and A. terreus, respectively. Co+2 and Ca+2 stimulated the enzyme activity of alkaline and acid protease s from A. terreus and acid proteases from A. niger. The other six cati ons and DTT inhibited variably the three proteases particularly alkali ne proteases from A. terreus indicating that proteases from various so urces even from closely related species showed different properties.