PHYTOSTEROL BIOSYNTHESIS - ISOTOPE EFFECTS ASSOCIATED WITH BIOMETHYLATION FORMATION TO 24-ALKENE STEROL ISOMERS

Authors
GUO DA JIA ZH NES WD
Citation
Da. Guo et al., PHYTOSTEROL BIOSYNTHESIS - ISOTOPE EFFECTS ASSOCIATED WITH BIOMETHYLATION FORMATION TO 24-ALKENE STEROL ISOMERS, Tetrahedron letters, 37(38), 1996, pp. 6823-6826
Citations number
11
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
37
Issue
38
Year of publication
1996
Pages
6823 - 6826
Database
ISI
SICI code
0040-4039(1996)37:38<6823:PB-IEA>2.0.ZU;2-0
Abstract
Changes in 24-alkene sterol product distribution resulting from deuter ium substitution on the coenzyme methyl group of AdoMet and on the ste rol acceptor molecule at C-23 and C-24 were used to determine kinetic isotope effects for the terminating deprotonations involved in sterol biomethylation catalyzed by (S)-adenosyl-L-methionine-Delta(24)-sterol methyl transferase (SMT) enzyme. By this method 24(28)-methylene cycl oartanol and cyclosadol were shown to be synthesized by two different SMT enzymes. Copyright (C) 1996 Published by Elsevier Science Ltd