H. Chart et al., HEAT-STABLE SEROTYPING ANTIGENS EXPRESSED BY STRAINS OF CAMPYLOBACTER-JEJUNI ARE PROBABLY CAPSULAR AND NOT LONG-CHAIN LIPOPOLYSACCHARIDE, Journal of Applied Bacteriology, 81(6), 1996, pp. 635-640
The role of lipopolysaccharide (LPS) in the serotyping of Campylobacte
r jejuni based on heat-stable antigens was examined using SDS-PAGE and
a silver strain for carbohydrate. None of the 32 type strains of Camp
. jejuni expressed long-chain LPS. Rabbit antibodies, prepared to 10 s
elected strains of Camp. jejuni, reacted with surface-exposed carbohyd
rate antigens, which were not LPS. This study suggests that the heat-s
table antigens of Camp. jejuni, which form the basis for the establish
ed Penner serotyping scheme, are probably capsular and not LPS.