FORMATION OF FLUOROPHORE OF YEAST D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE IN THE SOLUTION OF POTASSIUM-IODIDE

Authors
HE RQ LIU Y
Citation
Rq. He et Y. Liu, FORMATION OF FLUOROPHORE OF YEAST D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE IN THE SOLUTION OF POTASSIUM-IODIDE, Biochemistry and molecular biology international, 37(4), 1995, pp. 805-811
Citations number
17
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
37
Issue
4
Year of publication
1995
Pages
805 - 811
Database
ISI
SICI code
1039-9712(1995)37:4<805:FOFOYD>2.0.ZU;2-X
Abstract
A new fluorescence property of yeast D-glyceraldehyde-3-phosphate dehy drogenase was detected after it had been incubated in KI solution. Til e fluorophore formed in Baker's yeast GAPDH fluoresced at 383 nm by ex citation at 336 nm. The formation of tile fluorophore depended on NAD: Modification of Cys-149 with iodoacetic acid influenced some properti es of the fluorescence. It appears that the fluorophore may be situate d at or near the active site.