SUB-TYPING OF ANIMAL AND HUMAN CAMPYLOBACTER SPP USING RAPD

Citation
Rh. Madden et al., SUB-TYPING OF ANIMAL AND HUMAN CAMPYLOBACTER SPP USING RAPD, Letters in applied microbiology, 23(3), 1996, pp. 167-170
Citations number
18
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
02668254
Volume
23
Issue
3
Year of publication
1996
Pages
167 - 170
Database
ISI
SICI code
0266-8254(1996)23:3<167:SOAAHC>2.0.ZU;2-0
Abstract
Based on a 10-mer primer (5'-CCTGTTAGCC-3'), a random amplified polymo rphic DNA (RAPD) method for typing Campylobacter coli isolated from pi gs was developed. The method proved effective with a high discriminati on and good reproducibility. In contrast with serotyping no untypable strains were found out of a total of 269 isolates (veterinary, food an d clinical) examined. The method was also successfully applied to typi ng Campylobacter jejuni from a similar range of sources.