IMMUNOREACTIVITIES OF PKC-DELTA,ETA,ZETA IN BAKERS-YEAST, LOBSTER ANDWHEAT-GERM

Varied immunoreactive bands of protein kinase C delta (PKC delta), PKC eta, and PKC zeta were detected in crude extracts of wheat germ, lobs ter tail meat, and three strains of baker's yeast by analysis of Weste rn blots. Protease-deficient and Fleischmann's Active Dry yeasts exhib ited immunoreactivity of PKC delta, whereas wheat germ and Fleischmann 's RapidRise yeast displayed immunoreactivities of both PKC delta and PKC zeta. Lobster tail meat showed immunoreactivities of PKC eta and P KC zeta. These positive and negative immunoreactivities reflected evol utionary conservation and divergence, respectively, of these PKC isozy mes in eukaryotes.