ESTABLISHMENT OF VASOPRESSIN-RESPONSIVE EARLY PROXIMAL TUBULAR CELL-LINES DERIVED FROM TRANSGENIC MICE HARBORING TEMPERATURE-SENSITIVE SIMIAN-VIRUS-40 LARGE T-ANTIGEN GENE

A little is known about vasopressin receptor in early proximal tubule (S1). The purpose of this study is to establish the vasopressin-respon sive S1 cell line derived from transgenic mice harboring temperature-s ensitive(ts) simian virus (SV) 40 large T-antigen gene. The cells show ed a temperature-sensitive cell growth characteristic of encoding tsSV 40. The S1 cells retained a unique morphology specific to proximal tub ule. The cells showed the vasopressin-induced increase in intracellula r calcium concentration ([Ca2+]i) mediated by both V1a and the putativ e Vp receptor. Of nineteen clonal cell lines established from the pare ntal cells, three expressed only V1a receptor, and five retained both V1a and Vp receptor In conclusion, these immortalized S1 cell lines ma y be useful for studying vasopressin receptor subtypes in S1.