NF-KAPPA-B-MEDIATED REGULATION OF UROKINASE GENE-EXPRESSION BY PMA AND TNF-ALPHA IN HUMAN A549 CELLS

Binding sites for different transcription factors have been identified in the regulatory region of the human uPA gene, We investigated the r ole of NF-kappa B and AP-1 families of transcription factors in the in duction of uPA mRNA by TNF-alpha and PMA in the A549 cell line constit utively expressing uPA mRNA and protein, Using the protein synthesis i nhibitor cycloheximide and the antioxidant PDTC, that have an opposite effect on NF-kappa B and AP-1 activation, we showed that uPA mRNA ind uction by TNF-alpha and PMA in the A549 cell line is mainly due to NF- kappa B activation.