NOVEL HARMFUL EFFECTS OF [60]FULLERENE ON MOUSE EMBRYOS IN-VITRO AND IN-VIVO

[60]Fullerene (C60) was solubilized with poly(vinylpyrrolidone) (PVP) in water, and the aqueous solution was applied to a mouse midbrain cel l differentiation system, On incubation of C60 with various concentrat ions of PVP, cell differentiation and proliferation were potently inhi bited, although weaker than the vehicle controls, C60 was clearly dist ributed into the yolk sac and embryos by intraperitoneal administratio n to pregnant mice at 50 mg/kg and had a harmful effect on both concep tuses by microscopical evaluation, This in vivo and in vitro action on embryogenesis is a novel and seriously harmful activity of C60.