RAT-LIVER MITOCHONDRIA CAN SYNTHESIZE NICOTINAMIDE ADENINE-DINUCLEOTIDE FROM NICOTINAMIDE MONONUCLEOTIDE AND ATP VIA A PUTATIVE MATRIX NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE

Authors
BARILE M PASSARELLA S DANESE G QUAGLIARIELLO E
Citation
M. Barile et al., RAT-LIVER MITOCHONDRIA CAN SYNTHESIZE NICOTINAMIDE ADENINE-DINUCLEOTIDE FROM NICOTINAMIDE MONONUCLEOTIDE AND ATP VIA A PUTATIVE MATRIX NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE, Biochemistry and molecular biology international, 38(2), 1996, pp. 297-306
Citations number
30
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
38
Issue
2
Year of publication
1996
Pages
297 - 306
Database
ISI
SICI code
1039-9712(1996)38:2<297:RMCSNA>2.0.ZU;2-D
Abstract
In order to gain some insight into the mechanism by which nicotinamide nucleotides localize in mitochondria, NMN was added to rat liver mito chondria, with NAD synthesis tested both enzymatically and by means of HPLC. Evidence is given that the mitochondrial matrix contains a spec ific NMN adenylyltransferase (E.C. 2.7.7.1.), inhibited by PPi, AMP an d ADP-ribose. Some features of this enzyme, including the substrate, p H and temperature dependence were also investigated.