INTERACTION OF DIHYDROXYETHYLTHIAMINE PYROPHOSPHATE WITH TRANSKETOLASE

Authors
USMANOV RA SIDOROVA NN KOCHETOV GA
Citation
Ra. Usmanov et al., INTERACTION OF DIHYDROXYETHYLTHIAMINE PYROPHOSPHATE WITH TRANSKETOLASE, Biochemistry and molecular biology international, 38(2), 1996, pp. 307-314
Citations number
22
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
38
Issue
2
Year of publication
1996
Pages
307 - 314
Database
ISI
SICI code
1039-9712(1996)38:2<307:IODPWT>2.0.ZU;2-L
Abstract
Deprotonated form of dihydroxyethylthiamine pyrophosphate (DHETPP) is an intermediate product of the transketolase reaction. We have shown t hat when added to the reaction medium DHETPP can act as a substrate of the holoenzyme. The rate of the enzymatic reaction is quite close to that observed with ordinary substrates of transketolase. This fact sug gests that the interaction of DHETPP with the holotransketolase and it s deprotonation are not rate-limiting steps of the transketolase react ion.