CONTRIBUTION OF THE GLYCOLYTIC PATHWAY TO ENZYMATIC METMYOGLOBIN REDUCTION IN MYOCYTES

The metmyoglobin (oxidized form of myoglobin) reducing enzyme system w as examined in rat myocytes. An inhibitor of the glycolytic pathway (2 -deoxy-D-glucose) inhibited the reduction of metmyoglobin in myocytes. An inhibitor of the citric acid cycle (malonic acid), did not inhibit this reaction. From the results of this study along with those of our previous work, we proposed that the glycolytic pathway may have a sig nificant role in the enzymatic metmyoglobin reduction, presumably by i ts supply of cytoplasmic NADH for the NADH-cytochrome b(5) reductase s ystem, in muscle.