BINDING OF CANINE ANAPHYLACTIC ANTIBODIES ON HUMAN BASOPHILS - APPLICATION TO CANINE ALLERGY DIAGNOSIS

Using the technique of human basophil passive sensitization, as employ ed for human allergy diagnosis, we checked the ability of canine anaph ylactic antibodies to sensitize human basophils. Therefore, by sensiti zing human basophils with sera taken from dogs allergic to house dust mite, we demonstrated basophil activation as measured by alcian blue s taining. Basophil activation was inhibited by heating dog sera at 56 d egrees C for 6 h and by a human myeloma IgE. Basophil activation was a lso shown by histamine and leukotriene (LTC4) release. These results i ndicate canine anaphylactic antibodies bind to human basophil IgE rece ptors and also that they are IgE. The three methods described here for measuring basophil activation may lead to diagnostic methods applicab le to canine allergy diagnosis.