A NOVEL BACILLUS-INTERMEDIUS EXTRACELLULAR ALKALINE-PHOSPHATASE - ISOLATION, PHYSICOCHEMICAL AND CATALYTIC CHARACTERISTICS

Authors
SHARIPOVA MR BALABAN NP NEKHOTYAEVA NV MARDANOVA AM DEMENTIEV AA LESHCHINSKAYA IB
Citation
Mr. Sharipova et al., A NOVEL BACILLUS-INTERMEDIUS EXTRACELLULAR ALKALINE-PHOSPHATASE - ISOLATION, PHYSICOCHEMICAL AND CATALYTIC CHARACTERISTICS, Biochemistry and molecular biology international, 38(4), 1996, pp. 753-761
Citations number
16
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
38
Issue
4
Year of publication
1996
Pages
753 - 761
Database
ISI
SICI code
1039-9712(1996)38:4<753:ANBEA->2.0.ZU;2-E
Abstract
A new alkaline phosphatase was obtained as homogeneous preparation fro m culture filtrate of the spore-forming Bacillus intermedius. B. inter medius phosphatase was shown to be monomer with molecular weight of 47 kDa. The enzyme possesses phosphomonoesterase and phosphodiesterase a ctivities and exhibits a broad specificity towards a wide variety of s ubstrates. The purified phosphatase had an optimum temperature of 50 d egrees C, optimum pH of 9.5 and was stable until 60 degrees C at pH 8- 10. The effect of divalent metal ions and thiol reagents on catalytic activity of the enzyme was studied.