EGF RECEPTOR IN HUMAN CHANG LIVER AND HEPATOMA HEPG2 CELLS

Epidermal growth factor (EGF) receptor was detected in the human Chang liver and human hepatoma HepG2 cells. Both cell lines were found to b e able to bind EGF. The expression of EGF receptor in Chang liver and HepG2 cells was 1.3 x 10(5) EGF receptors/cell and 1.8 x 10(5) EGF rec eptors/cell, respectively. In both cells, this receptor was identified by ECL Western blotting using monoclonal anti-EGF receptor antibody a nd by immunohistochemical assay using polyclonal anti-EGF receptor ant ibody. Some of internalized EGF was recycled in Chang liver cells, but not in HepG2 cells.