REDUCTASE AND OXYGENASE ACTIVITIES OF SEMISYNTHETIC FLAVOCYTOCHROMES BASED ON CYTOCHROME-P450 2B4

Authors
SHUMANTSEVA VV UVAROV VY BYAKOVA OE ARCHAKOV AI
Citation
Vv. Shumantseva et al., REDUCTASE AND OXYGENASE ACTIVITIES OF SEMISYNTHETIC FLAVOCYTOCHROMES BASED ON CYTOCHROME-P450 2B4, Biochemistry and molecular biology international, 38(4), 1996, pp. 829-838
Citations number
24
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
38
Issue
4
Year of publication
1996
Pages
829 - 838
Database
ISI
SICI code
1039-9712(1996)38:4<829:RAOAOS>2.0.ZU;2-A
Abstract
A synthetic flavocytochrome with the reductase and oxygenase activitie s was obtained by covalent binding of riboflavin to cytochrome P450 2B 4. The reactions catalyzed by the newly synthesized flavocytochromes w ere studied. Formation of carboxycomplex with the reduced form of hemo protein led to 60-80% inhibition of oxygenase reactions indicating the leading role of reduced heme iron in generating active oxygen species by flavocytochromes.