THE SUBCUTANEOUS SPLEEN - A NEW MODEL FOR PERCUTANEOUS ACCESS TO THE PORTAL VENOUS SYSTEM

Correction of hepatic failure or metabolic disease by hepatocellular t ransplantation may require replacement of up to 10% of enzyme function with normal or genetically corrected cells. Although single injection s of hepatocytes have not been able to consistently achieve this level of functional replacement in experimental models, multiple injections should theoretically be able to approach this goal. Delivery of multi ple doses of hepatocytes to the spleen in experimental animals is comp licated by the need to perform multiple laparotomies. By relocating th e spleen to a subcutaneous position, the authors have designed an anim al model to facilitate multiple splenic injections without the need fo r repeated celiotomies. Because it is in the prefascial plane, multipl e hepatocyte injections can be delivered to the spleen percutaneously using only minimal sedation. Bleeding secondary to needle puncture is contained by a pseudocapsule which develops around the spleen. No-stat istical difference in the degree of hepatocyte migration to the liver has been demonstrated in animals transplanted via the subcutaneous spl een compared with animals transplanted by laparotomy (0.51% vs. 0.56%, p = .785).