USE OF THERMAL-ANALYSIS IN THE EVALUATION OF CRYOPRESERVATION PROTOCOLS FOR RIBES-NIGRUM L GERMPLASM

Citation
Eb. Benson et al., USE OF THERMAL-ANALYSIS IN THE EVALUATION OF CRYOPRESERVATION PROTOCOLS FOR RIBES-NIGRUM L GERMPLASM, Cryo-letters, 17(6), 1996, pp. 347-362
Citations number
18
Categorie Soggetti
Biology Miscellaneous",Physiology
Journal title
ISSN journal
01432044
Volume
17
Issue
6
Year of publication
1996
Pages
347 - 362
Database
ISI
SICI code
0143-2044(1996)17:6<347:UOTITE>2.0.ZU;2-B
Abstract
Shoot-tips of Ribes nigrum cultivars 'Ben Tron' and 'Ben More' were ca pable of surviving and regenerating new shoots following cryopreservat ion. Recovery responses were significantly enhanced when vitrification methods of cryopreservation were used. Differential scanning calorime try revealed that vitrification phenomena were reproducible and that i t was possible to stabilise the glassy state on rewarming. The potenti al for Ribes germplasm cryopreservation to be utilised in large scale conservation initiatives is evaluated in this study.