R. Bhushan et al., THE AMINO-ACID-SEQUENCE AND STRUCTURAL CLASS OF THE ARACHIN SUBUNIT OF MOLECULAR-WEIGHT 29,100A, Biochemistry and molecular biology international, 38(1), 1996, pp. 81-89
Arachin subunit (mol. wt. 29,100) from peanut of variety G-201 was sep
arated and isolated, its purity and homogeneity were ascertained. The
subunit was cleaved with trypsin, chymotrypsin and Staphylococcus aure
us V8 protease. The fragments obtained were separated and isolated by
PAGE, gel filtration & ion exchange chromatography, these were subject
ed to amino acid analysis, and Edman degradation. The PTH amino acids
obtained were identified by spectroscopy and TLC. The complete amino a
cid sequence of the subunit (226 residues) was established, and the st
ructural class of arachin was predicted from the amino acid compositio
n.