THE AMINO-ACID-SEQUENCE AND STRUCTURAL CLASS OF THE ARACHIN SUBUNIT OF MOLECULAR-WEIGHT 29,100A

Arachin subunit (mol. wt. 29,100) from peanut of variety G-201 was sep arated and isolated, its purity and homogeneity were ascertained. The subunit was cleaved with trypsin, chymotrypsin and Staphylococcus aure us V8 protease. The fragments obtained were separated and isolated by PAGE, gel filtration & ion exchange chromatography, these were subject ed to amino acid analysis, and Edman degradation. The PTH amino acids obtained were identified by spectroscopy and TLC. The complete amino a cid sequence of the subunit (226 residues) was established, and the st ructural class of arachin was predicted from the amino acid compositio n.