H-1-MAGNETIC RESONANCE SPECTROSCOPIC OBSERVATION OF CULTURED MALIGNANT-CELLS PHARMACOLOGICALLY INDUCED TO DIFFERENT PHENOTYPES

Rationale and Objectives. We evaluated the H-1 nuclear magnetic resona nce spectra of malignant cells after the administration of drugs that cause morphologic changes. Methods. H-1 spectra of a human lung adenoc arcinoma cell line cultured with interferon gamma, dexamethasone, or s odium butyrate were obtained. The peaks were assigned by two-dimension al homonuclear Hartmann-Hahn spectroscopy spectra of the cells and the ir perchloric acid extracts. Differential spectra were used to evaluat e relative changes in the peaks. Results. In the control culture, chol ine/phosphocholine peaks were increased in the cell-growth phase, and the 1.26-ppm peak was increased in the confluent state, Treatment by i nterferon gamma and dexamethasone induced reproducible changes in the peaks of differential spectra corresponding to 1.26 ppm, choline/phosp hocholine, and glutamate/glutamine. Dexamethasone treatment broadened lipid peaks. Changes after treatment with sodium butyrate were obscure . Microscopically, cells were induced to morphologically different phe notypes by each drug. Conclusion. Cells induced to exhibit morphologic ally different phenotypes present different H-1 spectra.