A SIMPLIFIED SEMIQUANTITATIVE DETERMINATION OF HEPATITIS-C VIRUS GENOME MOLECULES BY THE END-POINT DILUTION METHOD

Authors
BATHELIER C MERCIER G LUCOTTE G
Citation
C. Bathelier et al., A SIMPLIFIED SEMIQUANTITATIVE DETERMINATION OF HEPATITIS-C VIRUS GENOME MOLECULES BY THE END-POINT DILUTION METHOD, Molecular and cellular probes, 10(6), 1996, pp. 477-480
Citations number
12
Categorie Soggetti
Cell Biology",Biology,"Biochemical Research Methods
Journal title
Molecular and cellular probes → ACNP
ISSN journal
08908508
Volume
10
Issue
6
Year of publication
1996
Pages
477 - 480
Database
ISI
SICI code
0890-8508(1996)10:6<477:ASSDOH>2.0.ZU;2-X
Abstract
We describe a semiquantitative method to measure hepatitis C virus (HC V) viral particle numbers, by carrying out reverse-transcription polym erase chain reaction (RT-PCR) on serial dilutions of serum samples. Th e virus concentrations measured were 10(3)-10(6) viral particles ml(-1 ) of serum. The method described is relatively quick, and the only req uired manipulation is dilution of the serum. An optimal RT-PCR method is used for diluted and undiluted samples. (C) 1996 Academic Press Lim ited