ACCUMULATION OF PATHOGENESIS-RELATED PROTEINS IN BARLEY INDUCED BY PHOSPHATE AND SALICYLIC-ACID

Induction of pathogenesis-related proteins in intercellular spaces. of barley leaves was examined after inoculation with an incompatible rac e of powdery mildew (Erysiphe graminis f. sp. hordei) and salicylic ac id or phosphate treatment. Intercellular washing fluid was isolated fr om first leaf at pH 2.8 and proteins analysed by native polyacrylamid gel electrophoresis. Six major pathogen-induced bands (proteins) have been identified some of them were also induced by chemical treatment. In phosphate - sprayed leaves protein accumulation pattern was very si milar to that in powdery mildew inoculated leaves. Salicylic acid trea tment induced accumulation of two of the six pathogen-induced proteins and one salicylic acid specific protein. The behaviour of peroxidase, acid phosphatase, and non specific esterase were also investigated.