S. Declerck et al., ENTRAPMENT OF IN-VITRO PRODUCED SPORES OF GLOMUS VERSIFORME IN ALGINATE BEADS - IN-VITRO AND IN-VIVO INOCULUM POTENTIALS, Journal of biotechnology, 48(1-2), 1996, pp. 51-57
In vitro produced spores of Glomus versiforme were entrapped in algina
te beads and their inoculum potentials evaluated by a biological assay
. Encapsulated spores were able to germinate and the regenerated mycel
ium kept its ability to infect roots under controlled conditions. We e
stimated to 65.8 the number of beads needed, each containing four spor
es, to infect 50% of a population of susceptible plantlets. These resu
lts show the possibility to encapsulate in vitro produced spores and t
herefore represent a new kind of high quality inoculum, free of pathog
ens.