ANALYSIS OF KINETIC DATA OF ANTIBODY-ANTIGEN INTERACTION FROM AN OPTICAL BIOSENSOR BY EXPONENTIAL CURVE-FITTING

An optical biosensor system employing a resonant mirror (RM), with a s tirred cuvette has been used to follow the interaction of a recombinan t antibody fragment with its antigen, hen egg lysozyme. The data gener ated by the biosensor were analysed in order to determine the kinetic constants for the interaction using a linear transform (derivative ana lysis). For comparison the data were also analysed using an exponentia l curve fitting routine. It was demonstrated that the exponential curv e fitting method produced results which were in agreement with the exi sting linear transform method. It was also shown that early fitting of the association phase response, using the exponential curve fitting r outine between 0 and 70 s after sample addition, yielded sufficient in formation to provide a prediction of k(on). The potential use of the o ptical biosensor for the rapid monitoring of protein production and pu rification is discussed.