TREATMENT WITH RECOMBINANT BOVINE INTERFERON-TAU IN-UTERO ATTENUATES SECRETION OF PROSTAGLANDIN-F FROM CULTURED ENDOMETRIAL EPITHELIAL-CELLS

Endometrial oxytocin receptors and total production of PGF by endometr ial epithelial cells were measured in 10 cyclic cows after intrauterin e injections of recombinant bovine interferon-tau plus BSA or BSA alon e. Cows received twice daily injections (via intrauterine catheters) o f 200 mu g of recombinant bovine interferon-tau plus 1.3 mg of BSA (n = 5) or 1.5 mg of BSA (n = 5) from d 14 to 17 after estrus. On d 17, t he reproductive tracts of each cow was removed at slaughter, and endom etrial epithelial cells were cultured with 0, 2, or 50 ng/ml of recomb inant bovine interferon-tau. After 24 h, oxytocin (2 x 10(-7) M) was a dded to one-half of the culture wells, and the medium was sampled at 0 , 30, and 90 min for analysis of total PGF (PGF plus 13,14-dihydro-15- keto-PGF(2 alpha)). In vivo treatment with recombinant bovine interfer on-tau + BSA reduced total secretion of PGF in culture (1.49 +/- 0.06 vs. 2.80 +/- 0.07 ng/mu g of DNA), but did not block the oxytocin-indu ced stimulation in total secretion of PGF. In vitro treatment of cells with recombinant-bovine interferon-tau did not decrease basal secreti on of total PGF. Oxytocin receptor binding at d 17 was low in both tre atments but slightly attenuated in the group treated with recombinant bovine interferon-tau.