A HERPES-SIMPLEX VIRUS TYPE-1 MUTANT WITH A DELETION IMMEDIATELY UPSTREAM OF THE LAT LOCUS ESTABLISHES LATENCY AND REACTIVATES FROM LATENTLY INFECTED MICE WITH NORMAL KINETICS

The latency associated transcripts (LATs) are the only abundant viral gene products detected during latent herpes simplex virus (HSV) infect ion of peripheral nerves in animals and people. A LAT promoter has bee n identified and mutant viruses with lesions removing the promoter and surrounding region have been observed to reactivate slowly from trige minal ganglia (TG) explanted from latently infected mice. Previous wor k has shown that most mutants with lesions limited to regions downstre am of the LAT promoter reactivate normally. Therefore, to help map the boundaries of the slow reactivation phenotype, a mutant virus with le sions located immediately upstream of the LAT promoter was constructed and called 17 Delta S/N. 17 Delta S/N contains a 437 nucleotide (nt) deletion 332 nts upstream of the TATAA box of the LAT promoter, In pro ductively infected cells, 17 Delta S/N failed to synthesize detectable amounts of the 1.1 and 1.8 kb transcripts which are produced during w ild-tgpe infections and are specified by a region just upstream of the LAT promoter, However, 17 Delta S/N did produce normal amounts of LAT in tissue culture as well as ill neurons derived from latently infect ed cells, as ascertained by Northern blot and in situ hybridization an alysis. Moreover, in latently infected mice, 17 Delta S/N established and maintained infection in as many neurons as did wild type virus, as determined by in situ polymerase chain reaction (PCR) to deject viral DNA. Final ly, the virus reactivated from TG derived from latently in fected mice with kinetics indistinguishable from those of wild-type vi rus, Therefore, reactivation from latency, in this model system, does not appear to require function from the viral genomic region located i mmediately upstream of the LAT promoter.