BROMOPEROXIDASE AND IODOPEROXIDASE ENZYMES AND PRODUCTION OF HALOGENATED METHANES IN MARINE DIATOM CULTURES

Halogenated methanes produced in the oceans are important as carriers of chlorine, bromine, and iodine into the atmosphere. There they play roles in the regulation of ozone in the stratosphere and perhaps in th e Arctic troposphere at polar sunrise. While the mechanisms for the pr oduction of some polyhalogenated compounds by marine macrophytes have previously been substantially elucidated, the same has not been true i n the case of marine phytoplankton. We describe laboratory experiments on the production of various brominated and iodinated compounds in cu ltures of marine diatoms, obtained from the Provasoli-Guillard Center for Culture of Marine Phytoplankton collections (Bigelow Laboratory fo r Ocean Sciences, Maim, USA; CCMP). Species examined included Nitzschi a sp. (CCMP 580), Nitzschia arctica, Porosira glacialis, and two Navic ula sp. (CCMP 545 and 546). A suite of brominated compounds, notably b romoform and dibromomethane, is produced by the Nitzschia and Porosira species. Nitzschia sp.(CCMP 580) was grown In sufficient quantities t o allow the identification of a bromoperoxidase enzyme, which is assum ed to be responsible not only for the CHBr3 and CH2Br2 production but also for CH2I2 which was measured in those cultures. Chloroiodomethane was produced, either directly by the algae or by a photochemical reac tion of CH2I2. One Navicula species (CCMP 545), found to produce CH2I2 and CH2ClI, was shown to possess an iodoperoxidase. Bromoform and dib romomethane were not detected in cultures of this species. Other compo unds produced in certain of these non axenic cultures included methyl and ethyl iodide, and bromoiodomethane.