E. Bucci et al., PRODUCTION AND CHARACTERISTICS OF AN INFUSIBLE OXYGEN-CARRYING FLUID BASED ON HEMOGLOBIN INTRAMOLECULARLY CROSS-LINKED WITH SEBACIC ACID, The Journal of laboratory and clinical medicine, 128(2), 1996, pp. 146-153
Citations number
12
Categorie Soggetti
Medical Laboratory Technology","Medicine, General & Internal
Research on red cell substitutes requires the availability of oxygen-c
arrying fluids for physiologic experiments. This article describes the
procedure for in-house production of such a fluid. It coniains a hemo
globin-based oxygen carrier obtained by reacting human hemoglobin with
bis-(3,5-dibromosalicyl) sebacate. This reagent produces intramolecul
ar cross-links between the beta 82 lysines and between the alpha 99 ly
sines, respectively. The oxygen half-saturation pressure (P-50) of the
fluid is near 34 mm Hg at 37 degrees C, with a Hill's parameter of n=
2.2. The half-time of intravascular retention is near 3.0 hours in the
rat and 6.5 hours in the cat. Spectrophotometric analyses of arterial
and venous plasma from an infused rat reveal an efficient oxygen deli
very to the tissues by the oxygen carrier. Therefore, this new cross-l
inked human hemoglobin can be produced in quantities sufficient for in
vivo evaluation and with an oxygen affinity and cooperativity adequat
e for oxygen unloading in plasma.