LIPID-COMPOSITION OF THE GRAM-NEGATIVE, BUDDING, SEAWATER BACTERIUM HYPHOMONAS-JANNASCHIANA LACKING IN PHOSPHOLIPIDS

The extractable polar lipids of the gram-negative, budding, seawater b acterium Hyphomonas jannaschiana VP-2(T) were shown to of five glycoli pids, namely, 1,2-diacyl-3-alpha-D-glucopyranosyl-sn-glycerol (I) (25% of the total lipids), ,2-diacyl-3-alpha-D-glucuronopyranosyl-sn-glyce rol (II) (41%), taurine-amide of II (III) (32%), acyl-D-glucuronopyran oside (IV) (0.9%), and acyl-3-O-acyl-D-glucuronopyranoside (V) (0.4%); glycolipid (III) was described by the authors previously. In addition , small amounts of poly(beta-hydroxybutyrate) and free fatty acids wer e present in the Lipids extracted. Neither phospholipids nor low polar ity lipids, such as fatty acid esters of glycerol or fatty alcohols, w ere detected. Glycolipids I, II, IV, V were isolated in chromatographi cally pure state by chromatography on DEAE-cellulose and silica-gel co lumns. Their structures were established using chromatographic, spectr ometric and chemical-degradation methods. In glyceroglycolipids I and II, unsaturated fatty acid residues were located predominantly at the sn-1 carbon of the glycerol moiety while saturated ones were linked ma inly to the sn-2 carbon.