R. Sekido et al., ORGANIZATION OF THE GENE ENCODING TRANSCRIPTIONAL REPRESSOR DELTA-EF1AND CROSS-SPECIES CONSERVATION OF ITS DOMAINS, Gene, 173(2), 1996, pp. 227-232
delta EF1 (delta-crystallin/E2-box factor 1) is a widely distributed r
epressor of transcription which binds at the E2-box sequence, CACCTG,
It carries seven zinc fingers (Zf) in two clusters and a homeodomain i
n the middle as potential DNA-binding domains, We cloned the genomic g
ene encoding chicken delta EF1 and analyzed its organization, The gene
consisted of nine exons, the N-proximal Zf were encoded by exons 5 th
rough 7, and the C-proximal Zf by exons 8 and 9. Exon 7 also coded for
the large middle portion of the protein including the homeodomain. Pr
omoter analysis and RNase-protection assay indicated that the gene is
driven by a G+C-rich promoter without a TATA box, and the transcriptio
n start points (tsp) cluster around 20 bp from the start codon located
in exon 1, cDNA and genomic sequences of the mouse delta EF1 were clo
ned and compared with the chicken sequence, The deduced amino acid (aa
) sequence was highly conserved between the chicken and mouse delta EF
1, not only in DNA-binding motifs but also in other blocks (78% overal
l aa identity), More recently reported DNA-binding proteins, AREB6 (hu
man), ZEE (human) and BZP (hamster), were attributed to homologues of
delta EF1, among which only AREB6 represented a full-length sequence,
It was also indicated that rodent delta EF1 lacked exon 3.