A cDNA (hSPO) encoding human salivary peroxidase (hSPO) was isolated a
nd its nucleotide (nt) sequence determined. The coding sequence resemb
les those of other mammalian peroxidases and contains a 3' untranslate
d region of 538 nt, In a rabbit reticulocyte lysate transcription/tran
slation system, the cDNA produces a major protein product of approx, 6
7 kDa, which corresponds to the calculated molecular weight of unglyco
sylated hSPO.