CLONING AND SEQUENCING OF THE CDNA-ENCODING BETA-GLUCOSIDASE-1 FROM ASPERGILLUS-ACULEATUS

Authors
KAWAGUCHI T ENOKI T TSURUMAKI S SUMITANI J UEDA M OOI T ARAI M
Citation
T. Kawaguchi et al., CLONING AND SEQUENCING OF THE CDNA-ENCODING BETA-GLUCOSIDASE-1 FROM ASPERGILLUS-ACULEATUS, Gene, 173(2), 1996, pp. 287-288
Citations number
15
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
173
Issue
2
Year of publication
1996
Pages
287 - 288
Database
ISI
SICI code
0378-1119(1996)173:2<287:CASOTC>2.0.ZU;2-O
Abstract
A cDNA was isolated from an Aspergillus aculeatus cDNA library using s ynthetic oligodeoxyribonucleotide mixtures that corresponded to the in ternal amino acid (aa) sequence of mature beta-glucosidase 1 (BGL1). A nalysis of the nucleotide sequence of the cloned cDNA insert revealed a 2580-bp open reading frame (ORF) that encoded a 860-aa protein. The deduced aa sequence of the ORF shared sequence similarity with several BGL from other microorganisms.