T. Alacam et al., LACTATE-DEHYDROGENASE ACTIVITY IN PERIODONTAL-LIGAMENT CELLS STORED IN DIFFERENT TRANSPORT MEDIA, Oral surgery, oral medicine, oral pathology, oral radiology and endodontics, 82(3), 1996, pp. 321-323
Citations number
14
Categorie Soggetti
Pathology,Surgery,"Dentistry,Oral Surgery & Medicine
Background, Maintaining viability of periodontal ligament cells is imp
ortant after an avulsion injury occurs. Objective. This study examined
cell death and compared different transport media at varying times. L
actate dehydrogenase (LD) measurements were made on root surfaces of e
xtracted teeth for evaluating the results of breakdown and necrosis of
periodontal ligament cells. Study Design. Teeth were divided into thr
ee groups. Each was immersed in one of the following storage media: Ha
nks balanced salt solution, Custodiol solution (an organ storage mediu
m), or sterile saline solution. LD measurements (an indicator of cell
death) were made at 2, 6, 24, 72, and 168 hours after extraction. Resu
lts. When the LD levels were compared according to the time periods, a
progressive increase of cell death was found to be statistically sign
ificant (p < 0.001). However, when the effects of both time periods an
d the kind of storage medium on the LD levels were examined, the LD le
vels of the saline solution storage medium were found to be significan
tly higher (p < 0.001) than that of the other two groups. Conclusions,
Both Hanks balanced salt Solution and Custodiol appeared to be suitab
le transport media for maintaining cell viability, whereas saline solu
tion was not.