PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO BARLEY YELLOW MOSAIC-VIRUS AND THEIR USE IN DETECTION OF 4 BYMOVIRUSES

Six monoclonal antibodies (MAbs) against a French isolate of barley ye llow mosaic virus (BaYMV) pathotype 2 were produced and their isotypes determined. These MAbs were compared in ELISA for their reactivity wi th different isolates of BaYMV, wheat yellow mosaic virus (WYMV), whea t spindle streak mosaic virus (WSSMV) and oat mosaic virus (OMV). The six MAbs detected BaYMV in TAS ELISA and western blot, whereas in ACP ELISA no reaction was observed with isolates of BaYMV and WYMV. These MAbs could recognize the sequential motifs situated at the surface of viral particles. The six MAbs detected all the European isolates of Ba YMV pathotype 1 and 2 and the Japanese isolate of this viral pathotype I-1. In contrast to other MAbs, MAb IV did not react with the Japanes e isolate of BaYMV pathotype II-1. In TAS ELISA. MAbs I, II, III, and IV detected the Japanese isolate of WYMV and American isolates of WSSM V only when they were captured by anti-WYMV polyclonal antibodies. A F rench isolate of OMV was detected only by the MAbs I and II in TAS ELI SA with Polyclonal anti-BaYMV.