EFFECT OF ROGOR (30-PERCENT W W DIMETHOATE) ON THE ACTIVITY OF LACTATE-DEHYDROGENASE ACID AND ALKALINE-PHOSPHATASE IN MUSCLE AND GILL OF A FRESH-WATER FISH, HETEROPNEUSTES-FOSSILIS/

The effect of Rogor (30% W/W Dimethoate) on the activity of lactate de hydrogenase (EC. 1.1.1.27). Acid (EC. 3.1.32) and alkaline phosphatase (EC. 3.1.31) in muscle and gills of H. fossilis of both sexes were st udied. A significant decreas in the activities of acid and alkaline ph osphatase in the muscle and gills of both sexes was observed following sublethal exposure to Rogor except acid phosphatase of gills which sh owed increased activity in both the sexes. In contrast the activity of lactate dehydrogenase decreased in both the tissues during initial st age of exposure (5 days) whereas the activity of lactate dehydrogenase increased significantly during later stage (10 days) of exposure to R ogor in both sexes suggesting shift towards operation of anaerobic gly colytic pathway. The amount of protein in muscles and gills of male an d female fish decreased following Rogor exposure. The findings are dis cussed in relation to metabolism of fish during stress.