ONLINE HPLC-HRGC COUPLING AND SIMULTANEOUS TRANSFER OF 2 DIFFERENT LCFRACTIONS - DETERMINATION OF ALIPHATIC-ALCOHOLS AND STEROLS IN OLIVE OIL

A modified loop-type interface is described which uses two 6-way valve s and concurrent eluent evaporation to perform an on-line transfer and simultaneous gas chromatographic analysis of two different fractions pre-separated by liquid chromatography. The interface is used to simul taneously analyze aliphatic alcohols and sterols present in olive oil, LC pre-separation is carried out using a normal phase column (20 cm x 0.21 cm i.d.) and hexane-isopropanol (99:1) as a mobile phase at a fl ow of 0.2 ml/min; for the GC analysis a 5% phenyl, 95% dimethyl siloxa ne (25 m x 0.32 mm i.d., 0.4 mu m film thickness) column is used.