FRACTIONATION OF PROTEINS FROM WHEY WITH DIFFERENT PILOT-SCALE PROCESSES

Pilot scale production of alpha-lactalbumin (alpha-La) and beta-lactog lobulin (beta-Lg) from sweet and acid casein wheys rising two heat pre cipitation and two chromatographic methods was studied. Using the heat precipitation methods, the soluble beta-Lg was recovered easily by ul trafiltration, but the recovery of the low density alpha-La precipitat e was difficult. In addition, with acid casein whey, significant denat uration of alpha-La occurred. beta-Lg was selectively removed from aci d casein whey with strongly basic silica and polystyrenic anion exchan ge resin columns by elution with 0.1 mol/L HCl or 0.33 mol/L NaCl solu tion. beta-Lg eluted with HCl was highly denatured.