TLC CHARACTERIZATION OF SMALL UNILAMELLAR LIPOSOMES CONTAINING D-MYO-INOSITOL DERIVATIVES

The thin-layer chromatographic (TLC) behaviour of small unilamellar li posomes containing inositol phosphates (IPs) was studied. The vesicles contained different concentrations of D-myo-inositol 1,4,5-triphospha te (IP3), D-myo-inositol 1,2,6-triphosphate (alpha-trinositol, PP 56, a novel Perstorp Pharma derivative), D-myo-inositol 1,3,4,5-tetraphosp hate (IP4), D-myo-inositol 1,3,4,5,6-pentakisphosphate (IP5) and D-myo -inositol 1,2,3,4,5,6-hexakisphosphate (IP6). Migration of all liposom e batches was compared to that of control liposomes (multilamellar and small unilamellar, both containing only triple-distilled water), and to that of free phosphatidylcholine (PC). The same amount of lipid was used in all situations. Thin-layer chromatography was performed with silica gel as adsorbent. The developing solvent was an n-buthanol:etha nol:water mixture in a 4:3:3 volume ratio. At doses higher than 10(-2) M liposomes containing alpha-trinositol and IP6 had a different migra tion than PC, MLV or SUV as well as all batches of liposomes. Physiolo gical studies (using as model endothelized rat aorta rings) proved tha t in this situation they had no effects.