ENVIRONMENTAL METHODS FOR CRYPTOSPORIDIUM

This report was prepared by the Working Group on Waterborne Cryptospor idiosis (Technical Task Force E, Developmental Status of Environmental Sampling, Water Testing, and Surrogate Indicators). Methods for detec ting Cryptosporidium oocysts in water have centered around microscopic examination of fluorescent antibody-stained concentrates from large-v olume water samples. The limitations of these antibody-based methods i nclude the need for experienced analysts, lengthy analytical time, exp ense, lack of specificity, erratic efficiency, low precision, and diff iculty in determining viability. A number of methods, assays, and proc edures that have the potential for ameliorating some of these limitati ons are currently being evaluated. How successful such processes will. be remains to be demonstrated by the scientific community.