A DATABASE OF MUTANTS AND EFFECTS OF SITE-DIRECTED MUTAGENESIS EXPERIMENTS ON G-PROTEIN-COUPLED RECEPTORS

A database system and computer programs for storage and retrieval of i nformation about guanine nucleotide-binding protein (G protein) -coupl ed receptor mutants and associated biological effects have been develo ped, Mutation data on the receptors were collected from the literature and a database of mutants and effects of mutations was developed, The G protein-coupled receptor, family A, point mutation database (GRAP) provides detailed information on ligand-binding and signal transductio n properties of more than 2130 receptor mutants. The amino acid sequen ces of receptors for which mutation experiments have been reported wer e aligned, and from this alignment mutation data may be retrieved, Alt ernatively, a search form allowing detailed specification of which mut ants to retrieve may be used, for example, to search for specific amin o acid substitutions, substitutions in specific protein domains or rep orted biological effects, Furthermore, ligand and bibliographic orient ed queries may be performed, GRAP is available on the Internet (URL: h ttp://www-grap.fagmed.uit.no/GRAP/homepage.html) using the World-Wide Web system. (C) 1996 Wiley-Liss, Inc.